Journal: Cell stem cell

Article Title: CD150 high bone marrow Tregs maintain hematopoietic stem cell quiescence and immune privilege via adenosine

doi: 10.1016/j.stem.2018.01.017

Figure Lengend Snippet: A. Allo- or syn-donor chimerism in the peripheral blood of FoxP3cre CXCR4fl/wt or FoxP3cre CXCR4wt/wt mice nine and 24 weeks after transplantation. Recipient mice (B6) received 3-Gy irradiation followed by intravenous injection of MHC-mismatched B10.A BM cells or syngeneic B6 SJL BM cells (2 × 107/each). Anti-CD8 mAb was intraperitoneally injected on day –1 and anti-mouse CD40L mAb on day 0. Experiment included four recipients/group. Data were reproduced by an additional experiment (five mice/group).

Article Snippet: Anti CD40L (MR-1) , BioXell , BE0017–1 RRID:AB_1107601.

Techniques: Transplantation Assay, Irradiation, Injection

Journal: Cell stem cell

Article Title: CD150 high bone marrow Tregs maintain hematopoietic stem cell quiescence and immune privilege via adenosine

doi: 10.1016/j.stem.2018.01.017

Figure Lengend Snippet: KEY RESOURCE TABLES

Article Snippet: Anti CD40L (MR-1) , BioXell , BE0017–1 RRID:AB_1107601.

Techniques: Recombinant, Reverse Transcription, Gene Expression, Activation Assay, Saline, Red Blood Cell Lysis, Blocking Assay, Software

Journal:

Article Title: PREVENTION OF CHRONIC REJECTION IN MOUSE AORTIC ALLOGRAFTS BY COMBINED TREATMENT WITH CTLA4-IG AND ANTI-CD40 LIGAND MONOCLONAL ANTIBODY 1 , 2

doi:

Figure Lengend Snippet: Microscopic examination of AO obtained at day 30 after transplantation from B10→+C3H recipients in which signaling through both the CD28/B7 and CD40/gp39 pathways was concurrently blocked. Unlike discrete blockage of costimulatory pathways, combined simultaneous treatment with a single dose of both CTLA4-Ig and MR-1 resulted in marked reduction of intimal thickening (A; Verhoeff-van Gieson’s stain), which was patchy and eccentric (A, inset, hematoxylin and eosin). Further amelioration in changes distinctive of CR were evident after three doses of CTLA4-Ig and MR-1 (8; Verhoeff-van Gieson’s stain); nevertheless, minimal endothelial denudation (B, inset; hematoxylin and eosin) was discernible. It is noteworthy that in the majority of the animals, complete abrogation of CR was observed after treatment with 10 doses of CTLA4-Ig and MR-1, as delineated in hematoxylin and eosin (C); Verhoeff-van Gieson (D)- and α-smA+ (E)-stained sections of allogeneic aortas harvested on day 30 after transplantation. Original magnification: A and B, ×100; C, ×400; D and E, ×200; insets, ×400.

Article Snippet: Anti-CD40L (gp39; MR-1), a hamster mAb specific for murine gp39, was purchased from TSD Bioservice (Newark, DE).

Techniques: Transplantation Assay, Staining